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Effect of the agitation on the adsorption and hydrolytic efficiency of cutinases on polyethylene terephthalate fibres

Paper ID Volume ID Publish Year Pages File Format Full-Text
18131 42712 2007 5 PDF Available
Title
Effect of the agitation on the adsorption and hydrolytic efficiency of cutinases on polyethylene terephthalate fibres
Abstract

The effect of agitation on adsorption, desorption and hydrolytic efficiency of a native and a genetically modified cutinase (L182A) on polyethylene terephthalate fibres is reported in this paper. The effect of mechanical agitation was studied using a shaker bath with orbital agitation and a Rotawash machine with vertical agitation with and without extra steel discs inside the reaction pots. The results obtained indicate that mechanical agitation combined with enzymatic action enhances the adsorption and activity of cutinases towards PET (polyethylene terephthalate) fibres. L182A showed higher adsorption than the native enzyme for all the levels of mechanical agitation. Lower units of L182A lead to similar yields of terephthalic acid formed in all levels of mechanical agitation. The highest increase of hydroxyl surface groups was found for the genetically modified L182A at the lowest level of mechanical agitation with a shaker bath. These results indicate that enzymatic functionalization of PET is favoured with a process with lower levels of mechanical agitation.

Keywords
Cutinase; Polyester; Adsorption; Mechanical; Agitation
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Effect of the agitation on the adsorption and hydrolytic efficiency of cutinases on polyethylene terephthalate fibres
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 7, 1 June 2007, Pages 1801–1805
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us