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Immobilized glucose oxidase as a catalyst to the conversion of glucose into gluconic acid using a membrane reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
18138 42713 2007 6 PDF Available
Title
Immobilized glucose oxidase as a catalyst to the conversion of glucose into gluconic acid using a membrane reactor
Abstract

The aim of this work is to evaluate the performance of glucose oxidase (GO) (EC.1.1.3.4) adsorbed on DOWEX®-anionic resins in a continuous process using a membrane reactor coupled with a UF (cut-off of 100 kDa) or a MF (a pore diameter of 5 μm) membrane. Tests with soluble GO were also performed. At 20-h steady-state-continuous operations (constant parameters: 30 °C, pH 5.5, 100 rpm, 2.5 mM glucose, D = 6 h−1 and 0.094 U of GO), the reaction rate (r) and the conversion glucose/gluconic acid yield (Y) differed according to the type of membrane coupled to the reactor and to the form of GO used. The highest r (3.64 mmol/h U) and Y of 79% were observed in the test carried out in a UF-membrane reactor using soluble GO, whereas the highest Y (90%) and r of 2.37 mmol/h U occurred in the test carried out in a MF-membrane using immobilized GO. No leakage of enzyme from the support was detected.

Keywords
Glucose oxidation; Gluconic acid; Membrane reactor; Immobilized glucose oxidase; Anionic-polystyrene-divinilbenzene beads
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1020–1025
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us