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Simple indicators of plasmid loss during fermentation of Bacillus thuringiensis

Paper ID Volume ID Publish Year Pages File Format Full-Text
18142 42713 2007 7 PDF Available
Title
Simple indicators of plasmid loss during fermentation of Bacillus thuringiensis
Abstract

In an industrial fermentation of Bacillus thuringiensis, it is important to verify the intactness of bacterial plasmid content in all steps of culture. Changes in some plasmid resident properties of this bacterium could be considered as the simple potential signs of changes in the plasmid content. For studying this matter, two Cry+ (mother strain) and Cry− phenotype strains of B. thuringiensis H14 were compared. They showed different properties in many studied levels. Facing the presence of seven plasmids in the Cry+ strain only one small plasmid was observed in the Cry− strain. Despite the ability of mother strain to form aggregates (Agr+), no aggregation was observed in the Cry− phenotype (Agr−). The colony morphology of Cry+ strain was R shape while Cry− formed S shape colonies. Cry− strain grew faster than Cry+ strain, but both of them were capable to do sporulation. The protein profile using SDS-PAGE showed the presence of a 130, 65, and 25 kDa Cry protein in the Cry+ strain whereas these proteins were absent in the Cry− strain. The Cry− was not toxic against Aedes aegypti and Culex pipiens larvae.

Keywords
Bacillus thuringiensis H14; Fermentation; Plasmid; Bioinsecticide production; Aggregation; δ-Endotoxin
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Simple indicators of plasmid loss during fermentation of Bacillus thuringiensis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1052–1058
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us