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Combinatorial enzymic synthesis for functional testing of phenolic acid esters catalysed by Candida antarctica lipase B (Novozym 435®)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18146 42713 2007 9 PDF Available
Title
Combinatorial enzymic synthesis for functional testing of phenolic acid esters catalysed by Candida antarctica lipase B (Novozym 435®)
Abstract

Phenolic acid esters have various potential uses as functional food ingredients, e.g., as antioxidants and preservative agents. Combinatorial mixtures of phenolic acid esters were generated by mixing several different alcohol and acid substrates in various combinations and esterifying enzymically. It was shown to be feasible to identify all the combinatorial products using LCMS and to separate the individual components by HPLC for separate bioassay. Ester mixtures were tested, in comparison with un-esterified substrate mixtures, for anti-microbial effects and found to be active against strains of human gut bacteria. Esterification did not increase the strength of the activity, but significant synergies, both positive and negative, between the components of different ester mixtures were found. Isolated components of a mixture had much less activity than the mixture itself. In tests for protection of cultured cells from H2O2-induced oxidative damage and consequent apoptosis, esterification could increase the strength of protection, possibly because of increased bioavailability of the more membrane-soluble hydrophobic esters, compared with the water-soluble substrates. The combinatorial enzymic approach to generation of synergistic mixtures of phenolic acid esters has potential to find synergistic combinations that would be missed by testing individual compounds and combining only the active compounds.

Keywords
Lipase; Enzymatic; Anti-microbial; Phenolic antioxidant; Phenolic acid; Apoptosis
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Combinatorial enzymic synthesis for functional testing of phenolic acid esters catalysed by Candida antarctica lipase B (Novozym 435®)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1078–1086
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us