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Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose

Paper ID Volume ID Publish Year Pages File Format Full-Text
18151 42713 2007 7 PDF Available
Title
Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose
Abstract

A method is presented for synthesizing thermo-, acid-stable oligosaccharides (TASO) from sucrose (2.5–4 M) using a dextransucrase prepared from Leuconostoc mesenteroides B-512FMCM. The degree of polymerization (DP) of oligosaccharides synthesized was from 2 to 11. TASO resisted hydrolysis of its glycosidic linkages at 140 °C and pH 6.0 for 1 h. It was stable at pH's ranging from 2 to 4 at 120 °C. These oligosaccharides effectively inhibited the formation of insoluble glucan, the growth and acid production of Streptococcus sobrinus. However, it stimulated the growth of probiotic organisms such as Bifidobaterium sp. TASO potentially can be used as sweeteners for the food and beverages where thermo- and acid-stable properties are required and as potential inhibitors of dental caries.

Keywords
Dextransucrase; Thermo-stable; Acid-stable; Oligosaccharides; Bifidobacteria; Streptococcus sobrinus; Dental plaque
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Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1117–1123
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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