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UV induced mutations in Acidianus brierleyi growing in a continuous stirred tank reactor generated a strain with improved bioleaching capabilities

Paper ID Volume ID Publish Year Pages File Format Full-Text
18154 42713 2007 5 PDF Available
Title
UV induced mutations in Acidianus brierleyi growing in a continuous stirred tank reactor generated a strain with improved bioleaching capabilities
Abstract

Use of microorganisms for bioleaching processes is commonly limited by low tolerance of extreme culture conditions such as temperature, pH, and pulp density. Adaptation to extreme conditions is the main approach for strain improvement. Traditional methods for breeding strains with tolerance of extreme condition are difficult to apply without genetic tools because of many of such strains are not readily purely cultivated in liquid or agar medium. Here, we developed a method using ultraviolet (UV) irradiation for mutation in a continuous stirred tank reactor (CSTR) for screening to improve the bioleaching performance of a poorly characterized strain of Acidianus brierleyi (A. brierleyi). An improved strain developed with our method exhibited a good tolerance to Cu2+ and a high bioleaching rate of chalcopyrite, which was four-fold better than that of a strain selected by traditional adaptation method. We also designed a countercurrent bioleaching process in stirred tanks in series, and copper recovery in 7–8 days at a 15% chalcopyrite pulp density was more than 90% in a two stage countercurrent reactor.

Keywords
Adaptation; UV mutation in CSTR; A. brierleyi; Countercurrent bioleaching process
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UV induced mutations in Acidianus brierleyi growing in a continuous stirred tank reactor generated a strain with improved bioleaching capabilities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1136–1140
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us