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Effect of temperature and pressure on the combined action of purified tomato pectinmethylesterase and polygalacturonase in presence of pectin

Paper ID Volume ID Publish Year Pages File Format Full-Text
18155 42713 2007 6 PDF Available
Title
Effect of temperature and pressure on the combined action of purified tomato pectinmethylesterase and polygalacturonase in presence of pectin
Abstract

As expected, noticeable depolymerization of highly esterified pectin (DE, 80%) catalyzed by tomato polygalacturonase was observed when active tomato pectinmethylesterase was present, which was not the case in absence of tomato pectinmethylesterase. Besides, an increase in polygalacturonase activity was observed up to an optimal pressure level (100–200 MPa) depending on the temperature under study. Only at higher pressures, the rate of enzymatic pectin depolymerization diminished. In presence of active tomato polygalacturonase a higher tomato pectinmethylesterase activity was observed than in absence of tomato polygalacturonase at atmospheric pressure. The pectinmethylesterase catalyzed pectin deesterification accelerates by increasing pressure up to 200 MPa in presence of tomato polygalacturonase. Higher pressures diminished the tomato pectinmethylesterase activity becoming even lower as compared to atmospheric pressure, which is in contrast with the pectinmethylesterase activity studies performed in absence of tomato polygalacturonase. In general, tomato polygalacturonase activity was influenced indirectly by the presence of active tomato pectinmethylesterase and vice versa during thermal and high-pressure treatment. The combination of 40 °C and 400 MPa represents an optimal condition to preserve or improve rheological properties of tomato based products, because at this condition tomato polygalacturonase activity is reduced while pectinmethylesterase activity is maintained.

Keywords
Tomato pectinmethylesterase; Tomato polygalacturonase; Temperature and pressure processing; Pectin
First Page Preview
Effect of temperature and pressure on the combined action of purified tomato pectinmethylesterase and polygalacturonase in presence of pectin
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1141–1146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering