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Synthesis of 2′-deoxyibosylnucleosides using new 2′-deoxyribosyltransferase microorganism producers

Paper ID Volume ID Publish Year Pages File Format Full-Text
18156 42713 2007 9 PDF Available
Title
Synthesis of 2′-deoxyibosylnucleosides using new 2′-deoxyribosyltransferase microorganism producers
Abstract

Bacillus coagulans CECT 12, Bacillus psychrosaccharolyticus CECT 4074, Lactobacillus sp. CECT 4219 and Psychrobacter immobilis CECT 4492 have been characterised, after a taxonomic screening, as nucleoside 2′-deoxyribosyltransferase (type II) (E.C. 2.4.2.6) producers. They are selective for 6-aminopurine compared to 6-oxopurines and for 2′-deoxyuridine compared to thymidine. The synthesis of 2′-deoxyribosylnucleosides is performed in Tris/HCl at T = 57 °C, lower than the 70 °C commonly used in the literature. These microorganisms do not produce hypoxanthine by decomposition of adenine nucleoside as described for other strains. 2′-Deoxyribosylnucleosides of 5-chloro- or 5-fluoruracil can be obtained in a one-pot reaction. The strains can be immobilized in calcium alginate or calcium pectate producing biocatalysts active and selective in the synthesis of 2′-deoxyadenosine at 57 °C with high yields and without secondary reactions. In the present study two psychrotrophic microorganisms are described for the first time, B. psychrosaccharolyticus and P. immobilis, which show high nucleoside 2′-deoxyribosyltransferase activity both in free conditions and immobilized in calcium pectate.

Keywords
Psychrotrophic microorganisms; Nucleoside 2′-deoxyribosyltransferases; 2′-Deoxyribosylnucleoside synthesis; Immobilized whole cells; Calcium pectate; Calcium alginate
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Synthesis of 2′-deoxyibosylnucleosides using new 2′-deoxyribosyltransferase microorganism producers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1147–1155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us