fulltext.study @t Gmail

Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability

Paper ID Volume ID Publish Year Pages File Format Full-Text
18158 42713 2007 7 PDF Available
Title
Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability
Abstract

The immobilization of trypsin, chymotrypsin and carboxypeptidase A using 4 and 10% glyoxyl-agarose beads at different times of incubation was investigated. Enzyme loadings of 30 mg/mL gel for trypsin and chymotrypsin, and 2 mg/mL gel for carboxypetidase A were used. Immobilization rates were very rapid in both supports and reactions were completed after 1 h of reaction. Final residual activities at these concentrations were around 60% for trypsin and chymotrypsin, and 50% for carboxypeptidase A. Comparison of the thermal stability of the soluble and immobilized enzymes revealed that immobilization by binding to 10% glyoxyl-agarose yielded the most stable enzymatic activities. Reaction with this support yielded immobilized trypsin, chymotrypsin, and carboxypeptidase A that were 4700, 10,000, and 1000 times more stable than the soluble enzymes, respectively. It was observed that the number of lysine residues that took part in the immobilization process was a consequence of the type of support and reaction time of the experimental conditions, and that the increasing of the thermal stability of the derivatives was correlated with a increasing number of lysines residues involved in a multipoint covalent attachment.

Keywords
Immobilized enzymes; Glyoxyl-agarose; Thermal stability; Trypsin; Chymotrypsin; Carboxypeptidase A
First Page Preview
Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1160–1166
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us