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Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
18166 42713 2007 8 PDF Available
Title
Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications
Abstract

An alkaliphilic protease-producing bacterium was isolated and identified as Vibrio fluvialis TKU005. Two surfactant-stable alkaline metalloproteases (FI and FII) were purified from the culture supernatant of shrimp shell powder containing medium (pH 10). The molecular masses of FI and FII were 41 kDa and 39 kDa, respectively, assayed by SDS-PAGE. The optimum pH, optimum temperature, pH stability, thermal stability, Km, and Vmax of FI and FII were (9, 60 °C, 7–9, 50 °C, 2.21 mg/mL, 0.26 U/mL) and (9, 60 °C, 6–9, 60 °C, 3.92 mg/mL, 0.27 U/mL), respectively. The protease activities of FI and FII could be activated by the surfactant SDS (1%). The fifth day culture supernatant enhanced the growth of Chinese cabbage (Brassica campestris). The enhancing effect was due to hydrolyzates of the metabolites rather than the proteases.

Keywords
Vibrio fluvialis; Protease; Shrimp shell; Chitin; Biofertilizer
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Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1213–1220
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us