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Increased laccase production by Trametes hirsuta grown on ground orange peelings

Paper ID Volume ID Publish Year Pages File Format Full-Text
18176 42713 2007 5 PDF Available
Title
Increased laccase production by Trametes hirsuta grown on ground orange peelings
Abstract

The present work focuses on the obtaining of high laccase activity levels by optimising several variables affecting laccase production under solid-state conditions. Thus, the effect of the addition of laccase-inducing compounds (syringaldazine, copper sulphate) and the amount of support utilised on laccase activity was studied. The highest activities (31,786 U/L) were obtained operating at the following conditions: 2.5 g ground orange peelings and 5 mM copper sulphate. The process was also scaled to 250 mL fixed-bed and 1.8 L tray reactors. The former produced maximal activities of about 3000 U/L, whereas the latter exhibited much higher values of around 12,000 U/L. To our knowledge, these activities are higher than those reported to date at bioreactor scale operating with a wild organism.

Keywords
Bioreactor; Laccase; Orange peelings; Solid-state fermentation; Trametes hirsuta
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1286–1290
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us