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Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
18177 42713 2007 9 PDF Available
Title
Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol
Abstract

To investigate the possible use of Saccharomyces cerevisiae as a candidate for consolidated bioprocessing (CBP) of cellulose to ethanol, four fungal cellobiohydrolase (CBH) encoding genes (Trichoderma reesei cbh1 and cbh2, Aspergillus niger cbhB and Phanerochaete chrysosporium cbh1–4) were expressed in this yeast. All four CBHs were successfully expressed and similar extracellular activity was demonstrated on phosphoric acid swollen cellulose (PASC) and bacterial microcrystalline cellulose (BMCC) using a modified affinity digestion procedure. Our results suggest that although heterologous CBHs can be produced in S. cerevisiae the titers of functionally secreted CBH are relatively low. However, the specific activity of recombinant CBH1 on PASC and BMCC, determined by a sensitive ELISA-based technique, was found not to differ significantly from that of the native T. reesei enzyme. Given this similarity in specific activity, but the disparity between current levels of CBH expression relative to expression levels required to enable growth on crystalline cellulosic substrates with concomitant ethanol production, future studies should aim to increase the expression levels of CBHs.

Keywords
Cellobiohydrolase; Cellulase expression in yeast; CBH specific activity; Consolidated bioprocessing
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Functional expression of cellobiohydrolases in Saccharomyces cerevisiae towards one-step conversion of cellulose to ethanol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1291–1299
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us