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Production of polyhydroxyalkanoates by Herbaspirillum seropedicae grown with different sole carbon sources and on lactose when engineered to express the lacZlacY genes

Paper ID Volume ID Publish Year Pages File Format Full-Text
18185 42713 2007 6 PDF Available
Title
Production of polyhydroxyalkanoates by Herbaspirillum seropedicae grown with different sole carbon sources and on lactose when engineered to express the lacZlacY genes
Abstract

Batch-fermentation experiments showed that Herbaspirillum seropedicae Z69 was able to accumulate up to 36% of its biomass as PHB when grown on glucose as a carbon source. Accumulation of PHB paralleled entry of the bacteria into the stationary phase of growth. When grown on nonanoic acid as a sole carbon source H. seropedicae accumulated poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV) with a relative abundance of 2:1 for the 3HB and 3HV monomers, respectively. Enzymatic activity specifying PHB biosynthesis, i.e., β-ketothiolase and acetoacetyl-CoA reductase (NADPH) were found in cell-free extracts of strains Z67 and Z69. To enable H. seropedicae to grow on lactose, a recombinant H. seropedicae strain, Z69 (pHM3) expressing the Escherichia coli lacZ lacY genes was constructed. H. seropedicae Z69 (pHM3) grown on lactose as a sole carbon source accumulated up to 36% of its biomass as PHB.

Keywords
Herbaspirillum seropedicae; Poly(3-hydroxybutyrate); Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); lacZlacY
First Page Preview
Production of polyhydroxyalkanoates by Herbaspirillum seropedicae grown with different sole carbon sources and on lactose when engineered to express the lacZlacY genes
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1352–1357
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering