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Purification and characterization of a novel ginsenoside-hydrolyzing β-d-glucosidase from the China white jade snail (Achatina fulica)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18186 42713 2007 9 PDF Available
Title
Purification and characterization of a novel ginsenoside-hydrolyzing β-d-glucosidase from the China white jade snail (Achatina fulica)
Abstract

A β-d-glucosidase, with broad regiospecific activity and designated as G II, was purified to homogeneity from the acetone power of viscera of the China white jade snail (Achatina fulica). G II consisted of two identical subunits (110 kDa) with a native molecular mass of 220 kDa. G II was stable over a wide pH range (4–10 at 30 °C for 24 h) and at a relatively high temperature (50 °C for 8 h). Moreover, it can cleave both β-(1 → 2)-glucosidic linkage at 3-C and β-(1 → 6)-glucosidic linkage at 20-C of ginsenosides and can hydrolyze ginsenosides Rb1, Rb2, Rb3 and Rc into their active metabolites, compound K, compound Y, Mx, and Mc, respectively. The optimal activity against p-nitrophenyl-β-d-glucopyranoside (pNPG) was at 50 °C and pH 5.0. Under the same condition, the Km for pNPG was 0.224 mM with a Vmax of 0.203 mmol nitrophenol min−1 mg−1. Of the substrates tested, G II specifically hydrolyzed the β-d-glucosides involving aryl-β-glucosides, alkyl-β-glucosides, and β-linked disaccharides (i.e. sophorose, gentiobiose, and cellobiose). These findings make the novel enzyme potentially useful in biotransformation processes as well as in the modification of multiple ginsenosides.

Keywords
β-d-Glucosidase; Enzyme purification; Ginsenosides; Compound K; China white jade snail
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Purification and characterization of a novel ginsenoside-hydrolyzing β-d-glucosidase from the China white jade snail (Achatina fulica)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1358–1366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us