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Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design

Paper ID Volume ID Publish Year Pages File Format Full-Text
18187 42713 2007 6 PDF Available
Title
Optimization of medium constituents for the production of citric acid from byproduct glycerol using Doehlert experimental design
Abstract

Citric acid production was maximized by Yarrowia lipolytica NCIM 3589 in submerged fermentation using byproduct glycerol obtained during biodiesel production, which is produced by the transesterification of oils obtained from the seeds of nonedible plants as the renewable carbon source. Response surface methodology (RSM) involving Doehlert experimental design was adopted to evaluate the amount of citric acid produced by most important factors, such as yeast extract, raw glycerol, and salt solution concentration. Besides reducing the number of experiments required for optimization, this technique allowed to quantify the amount of citric acid in any part of the experimental domain. The optimal set of conditions for maximum citric acid production was as follows: yeast extract 0.2682 g/l, raw glycerol 54.4081 g/l, and salt solution concentration 13.6936% (v/v). A maximum citric acid concentration of 77.3999 g/l was obtained at these optimal conditions.

Keywords
Citric acid; Byproduct glycerol; Submerged fermentation; Yarrowia lipolytica; Response surface methodology; Optimization; Doehlert experimental design
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1367–1372
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us