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Improvement of xylanase production by Penicillium oxalicum ZH-30 using response surface methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
18189 42713 2007 8 PDF Available
Title
Improvement of xylanase production by Penicillium oxalicum ZH-30 using response surface methodology
Abstract

The effects of substrate concentration, pH, and cultivation time on production of xylanase from Penicillium oxalicum ZH-30 were optimized by statistical analysis using response surface methodology. The response surface methodology (RSM) was used to optimize xylanase production by implementing the Box-Wilson design. Statistical analysis of the results showed that the linear and quadric terms of these three variables had significant effects. However, no interactions between the three variables were found to contribute to the response at a significant level. The optimal conditions for higher production of xylanase were wheat bran: 35.6 g/L, pH 7.72, and cultivation time: 142.3 h. Under these conditions, the model predicted a xylanase activity of 14.91 U/mL. Verification of the optimization showed that xylanase production of 14.50 U/mL was observed under the optimal conditions in the shake flasks experiment. The maximum xylanase activity reached 16.11 U/mL after 144 h cultivation in the 15-L scaled fermenter.

Keywords
Penicillium oxalicum ZH-30; Optimization; Response surface methodology; Xylanase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1381–1388
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us