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Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18194 42713 2007 5 PDF Available
Title
Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA)
Abstract

We succeeded in the immobilization of 190 mg of periodate oxidized glucoamylase per gram of macroporous polymer. The covalently immobilized enzyme had a specific activity of 1100 U/g. The temperature and pH optimum as well as kinetic parameters were determined. The immobilized enzyme was tested in different types of reactors for hydrolysis of concentrated maltose and starch hydrolysate syrups. The DE value of 98.6, obtained the immobilized enzyme, was slightly higher than that for the soluble form, when acting on 20% substrates.During continuous use in a packed bed reactor over a period of 4 weeks the immobilized enzyme produced 1300 kg of glucose per 1 L of reactor volume without any decrease in its activity.

Keywords
Amyloglucosidase; Glycidyl methacrylate; Immobilization; Periodate; Starch
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Immobilization of glucoamylase via its carbohydrate moiety on macroporous poly(GMA-co-EGDMA)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1422–1426
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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