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Biochemical characterisation of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6

Paper ID Volume ID Publish Year Pages File Format Full-Text
18196 42713 2007 7 PDF Available
Title
Biochemical characterisation of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6
Abstract

Two phenol oxidases, a tyrosinase and a laccase were isolated from cell extracts of the soil bacterium Pseudomonas putida F6. Both oxidases were purified to homogeneity separately using a combination of anion exchange chromatography, gel filtration and gel slicing. The purified tyrosinase is a monomer with a relative molecular mass (Mr) of approximately 39,000 while the purified laccase has a relative molecular mass (Mr) of approximately 59,000. Maximum activity, for tyrosinase with l-tyrosine (monophenolase) and l-dopa (diphenolase) and for laccase with syringaldazine, was observed at 30 °C and pH 7.0. Both enzymes were stable over a broad range of temperatures and were most stable at 30 °C. Both the monophenolase and diphenolase activities of tyrosinase were relatively stable across a broad range of pH values with maximum stability at pH 5.0 and 4.0, respectively. Laccase was most stable at pH 7.0 with a narrow bell shaped curve over a range of pH values. P. putida F6 tyrosinase has a 1.5-fold higher affinity for l-tyrosine compared to l-dopa. Furthermore P. putida F6 tyrosinase exhibits a 2.7-fold higher affinity for α-methyl-dl-tyrosine compared to α-methyl-l-tyrosine.

Keywords
Pseudomonas putida F6; Tyrosinase; Laccase
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Biochemical characterisation of the coexisting tyrosinase and laccase in the soil bacterium Pseudomonas putida F6
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1435–1441
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us