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The silica-coated chitosan particle from a layer-by-layer approach for pectinase immobilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
18197 42713 2007 6 PDF Available
Title
The silica-coated chitosan particle from a layer-by-layer approach for pectinase immobilization
Abstract

We investigate the enzymatic activity of pectinase adsorbed on a novel type of colloidal particles. The colloidal stability is not impeded by the adsorbed proteins despite the fact that up to 247.8 mg of enzyme is adsorbed per gram of the carrier particles. Various characteristics of immobilized pectinase such as the pH stability, thermal stability, and storage stability were valuated. The immobilized pectinase revealed acceptable pH stability over a broad experimental range. The activity of immobilized pectinase adsorbed onto these particles is analyzed in terms of the Michaelis–Menten parameters. The Michaelis constant Km differs only slightly from the Km value of the native enzyme when the amount of adsorbed enzyme is raised despite the high local concentration of immobilized enzymes.

Keywords
Chitosan; Layer by layer; Polyelectrolyte; Immobilized pectinase enzyme
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The silica-coated chitosan particle from a layer-by-layer approach for pectinase immobilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1442–1447
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us