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Phospholipid bilayer formation on a variety of nanoporous oxide and organic xerogel films

Paper ID Volume ID Publish Year Pages File Format Full-Text
1821 91 2011 7 PDF Available
Title
Phospholipid bilayer formation on a variety of nanoporous oxide and organic xerogel films
Abstract

Lipid bilayers supported by nanoporous xerogel materials are being explored as models for cell membranes. In order to better understand and characterize the nature of the surface–bilayer interactions, several oxide and organic nanoporous xerogel films (alumina, titania, iron oxide, phloroglucinol–formaldehyde, resorcinol–formaldehyde and cellulose acetate) have been investigated as a scaffold for vesicle-fused 1,2-dioleoyl-glycero-3-phosphocholine (DOPC) lipid bilayer formation and mobility. The surface topography of the different substrates was analyzed using contact and tapping-mode atomic force microscopy and the surface energy of the substrates was determined using contact angle goniometry. Lipid bilayer formation has been observed with fluorescence microscopy and lateral lipid diffusion coefficients have been determined using fluorescence recovery after photobleaching. Titania xerogel films were found to be a robust and convenient support for formation of a two-phase DOPC/1,2-distearoyl-glycero-3-phosphocholine bilayer and domains were observed with this system. It was found that the cellulose acetate xerogel film support produced the slowest lipid lateral diffusion.

Keywords
Lipid bilayer; Sol–gel; Surface energy; FRAP; AFM
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Phospholipid bilayer formation on a variety of nanoporous oxide and organic xerogel films
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 1, January 2011, Pages 380–386
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us