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Expression and improved production of the soluble extracellular invertase from Zymomonas mobilis in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
18210 42714 2006 6 PDF Available
Title
Expression and improved production of the soluble extracellular invertase from Zymomonas mobilis in Escherichia coli
Abstract

The extracellular invertase from Zymomonas mobilis (INVB) was used as a model protein to study the recombinant protein production by Escherichia coli. The effect of temperature, induction and post-induction time on expression of soluble INVB protein was studied in a bench-top fermenter. The maximum productivity of soluble protein (2.7 g l−1 h−1) was obtained by decreasing the temperature (0.05 °C min−1) from 37 to 27 °C after induction with IPTG at the beginning of stationary phase. Under these conditions, the productivity of soluble INVB protein was increased by factor of 10, in relation to the process carried out overall at 37 °C.

Keywords
Zymomonas mobilis; Invertase; Soluble recombinant protein; Production; Escherichia coli
First Page Preview
Expression and improved production of the soluble extracellular invertase from Zymomonas mobilis in Escherichia coli
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 1, 6 December 2006, Pages 61–66
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering