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KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4-lacZ-based cadmium and copper biosensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
18211 42714 2006 4 PDF Available
Title
KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4-lacZ-based cadmium and copper biosensor
Abstract

Based on the effects of cadmium and copper on KlHIS4 transcription when expressed in either Kluyveromyces lactis or Saccharomyces cerevisiae, a yeast biosensor is presented. It consists of a lacZ fused to a KlHIS4 447 bp long promoter expressed in K. lactis, and as control, a second fusion with a shorter promoter not regulated by metals. This eukaryotic system can be used to detect cadmium and copper concentrations of 0.3–3 mM (or higher) within minutes using X-GAL filter assay and other variants of β-galactosidase assays developed in multititer wells.

Keywords
Cadmium; Copper; Biosensor; Yeast
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KlHIS4-modulated histidine auxotrophy in yeast and characterisation of a KlHIS4-lacZ-based cadmium and copper biosensor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 1, 6 December 2006, Pages 67–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us