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Evaluating the genetic diversity of dioxygenases for initial catabolism of aromatic hydrocarbons in Pseudomonas rhodesiae KK1

Paper ID Volume ID Publish Year Pages File Format Full-Text
18212 42714 2006 8 PDF Available
Title
Evaluating the genetic diversity of dioxygenases for initial catabolism of aromatic hydrocarbons in Pseudomonas rhodesiae KK1
Abstract

Pseudomonas rhodesiae strain KK1 was found to be capable of utilizing a broad range of aromatic compounds as well as polycyclic aromatic hydrocarbons in this study. The Rieske-type iron–sulfur center sequences of dioxygenases obtained from KK1 through PCR process using a universal dioxygenase primer set were used to evaluate catabolic potential for aromatic compounds. Comparative analysis of predicted amino acid sequences from 50 randomly selected dioxygenase clones capable of hydroxylating inactivated aromatic nuclei indicated that the clones from strain KK1 could be divided into five groups. Amino acid sequences of each dioxygenase clone were a part of the genes encoding enzyme for initial catabolism of benzoate, carbazole, p-cumate, naphthalene, phenanthrene, biphenyl, or vanillate. Radiorespirometric analysis revealed that strain KK1 was able to mineralize polycyclic aromatic hydrocarbons such as anthracene, naphthalene and phenanthrene, as well as a nitrogen heterocyclic aromatic compound-like carbazole. Also, it has been found through HPLC analysis that benzoate, biphenyl, p-cumate, and vanillate also could be degraded by strain KK1. These results were consistent with data obtained through analyses of dioxygenase clones and substrate conversion assay, providing the evidence that strain KK1 has the capability to degrade a broad range of aromatic substrates including polycyclic aromatic hydrocarbons.

Keywords
Dioxygenases; Aromatic hydrocarbons; Radiorespirometry; Pseudomonas rhodesiae KK1
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Evaluating the genetic diversity of dioxygenases for initial catabolism of aromatic hydrocarbons in Pseudomonas rhodesiae KK1
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 1, 6 December 2006, Pages 71–78
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us