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Ganoderma lucidum inhibits tumour cell proliferation and induces tumour cell death

Paper ID Volume ID Publish Year Pages File Format Full-Text
18229 42714 2006 9 PDF Available
Title
Ganoderma lucidum inhibits tumour cell proliferation and induces tumour cell death
Abstract

Ganoderma lucidum, a traditional Chinese medicinal fungus, has been a favourite remedy in oriental medicine for centuries. The objective of this study is to analyze whether G. lucidum affects cancer cell proliferation and cell death. Malignant human breast carcinoma cells were used in our studies. Different preparations of G. lucidum spores were added to the cancer cells at a final concentration of 1 mg/ml followed by incubation of the cultures for two days. Treatment with G. lucidum resulted in tumour cells detachment from the tissue culture plates and death. The proliferation of the adherent cells was also inhibited. The experiments indicated that the inhibitory effects of G. lucidum on cancer cell growth were sporoderm-broken spores (broken by enzymatic method) > sporoderm-broken spores (broken by physical method) > intact spores > buffer control. The polysaccharides isolated from G. lucidum cultivated with wood-logs exhibited the greatest inhibitory effect on cell proliferation, which was concentration-dependent. These results were confirmed by trypan blue staining and MTT assay. The inhibitory effect of G. lucidum on cell proliferation appeared to occur through the Erk pathway: The expression of Erk was reduced in the presence of G. lucidum polysaccharide.

Keywords
DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; HBSS, Hank's balanced salt solution; PAGE, polyacrylamide gel electrophoresis; MTT assay, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenly tetrazolium bromide] assay; Erk, extracellular regu
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Ganoderma lucidum inhibits tumour cell proliferation and induces tumour cell death
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 1, 6 December 2006, Pages 177–185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us