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The effect of carbohydrate carbon sources on the production of cellulase by Phlebia gigantea

Paper ID Volume ID Publish Year Pages File Format Full-Text
18233 42715 2007 5 PDF Available
Title
The effect of carbohydrate carbon sources on the production of cellulase by Phlebia gigantea
Abstract

Phlebia gigantea is an aggressive white rot fungus that colonizes conifer wood. The influence of different carbohydrates: glucose, xylose, carboxymethyl cellulose (CMC), microcrystalline cellulose (avicel) and cellobiose were evaluated as carbon sources for the production of cellulase by this saprophytic fungus. Carboxymethyl cellulose gave the highest yield, followed by cellobiose and avicel. Glucose and xylose did not produce any detectable cellulases which suggest the repression of cellulase in presence of these simple carbohydrates by P. gigantea. These results showed that the production of cellulase not only depends on the pH but also on the consumption of carbon sources in medium. The higher yield of cellulase in presence of CMC, the amorphous cellulose, and less in presence of cellobiose the soluble intermediate product of cellulose hydrolysis, indicates the requirement of crystallinity and complexity for the higher yield of cellulase by P. gigantea.

Keywords
Cellulase; Phlebia gigantea; Substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 6, 2 May 2007, Pages 1464–1468
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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