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A novel biosensor for the detection and monitoring of β-d-galactosidase of faecal origin in water

Paper ID Volume ID Publish Year Pages File Format Full-Text
18240 42715 2007 6 PDF Available
Title
A novel biosensor for the detection and monitoring of β-d-galactosidase of faecal origin in water
Abstract

A voltammetric sensor prepared by the immobilization of metallophthalocyanine complexes onto a glassy carbon electrode has been developed for the detection of β-d-galactosidase (B-GAL) of faecal origin in water. Electrooxidation of chlorophenol red, a breakdown product of the chromogenic substrate chlorophenol red β-d-galactopyranoside, was used as a measure of β-d-galactosidase activity. At metallophthalocyanine modified electrodes, in particular copper(II) phthalocyanine, a decrease in electrode fouling was observed. The sensor was sensitive to fluctuations in pH, not significantly affected by temperature variations and could detect one colony forming unit/100 mL in 15 min. Loss of 40% sensitivity was observed over a period of 30 days. A strong correlation between sensor sensitivity and colony forming units was observed. The sensor is capable of detecting viable but nonculturable bacteria, overcoming this drawback of the use of culture media for detection of coliforms.

Keywords
Chlorophenol red; Chlorophenol red β-d-galactopyranoside; Coliforms; Voltammetry; Phthalocyanine; Biosensor
First Page Preview
A novel biosensor for the detection and monitoring of β-d-galactosidase of faecal origin in water
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 6, 2 May 2007, Pages 1512–1517
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering