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Comparison of different support materials for their capacity to immobilize Mycobacterium austroafricanum IFP 2012 and to adsorb MtBE

Paper ID Volume ID Publish Year Pages File Format Full-Text
18242 42715 2007 7 PDF Available
Title
Comparison of different support materials for their capacity to immobilize Mycobacterium austroafricanum IFP 2012 and to adsorb MtBE
Abstract

A strain of Mycobacterium austroafricanum capable of mineralizing methyl tert-butyl ether (MtBE) as its sole carbon and energy source had previously been isolated from activated sludge. The use of this bacteria in a biobarrier system for the in situ treatment of contaminated groundwater was the ultimate goal of this work. This paper reports the results of initial studies to find an appropriate immobilizing or support material. Several materials (peat, perlite, pozzolan, pumice and granulated activated carbon) were considered. Selection was based on the measurement and comparison of their abilities to immobilize or retain the bacteria, and to adsorb MtBE.The immobilization test was based on monitoring the respiration of bacteria remaining on the material after initial contact times of either 24 or 72 h. O2 consumption and CO2 production were measured over a period of time. The study showed that perlite was the best material.Adsorption isotherms for MtBE were constructed for each support. The strength of adsorption coefficient, Kf, in the Freundlich equation was calculated and used as the basis for comparison of their adsorptive capacities. The strength of adsorption was highest for the granulated activated carbon, and the Kf values for the other materials were relatively low and fell within the same range. However, since GAC performed poorly in the immobilization test, perlite was selected as the best support material.

Keywords
MtBE biodegradation; Mycobacterium austroafricanum IFP 2012; Immobilizing agent; Support material; Respiration; Adsorption
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Comparison of different support materials for their capacity to immobilize Mycobacterium austroafricanum IFP 2012 and to adsorb MtBE
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 6, 2 May 2007, Pages 1524–1530
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us