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Co-expression of l-arabinose isomerase and d-glucose isomerase in E. coli and development of an efficient process producing simultaneously d-tagatose and d-fructose

Paper ID Volume ID Publish Year Pages File Format Full-Text
18243 42715 2007 7 PDF Available
Title
Co-expression of l-arabinose isomerase and d-glucose isomerase in E. coli and development of an efficient process producing simultaneously d-tagatose and d-fructose
Abstract

To develop a feasible enzymatic process for the concomitant d-tagatose and d-fructose production, the thermostable l-arabinose isomerase of Bacillus stearothermophilus US100 (l-AI US100) and the mutant d-glucose isomerase obtained from that of Streptomyces SK (SKGI-A103G) were successfully co-expressed in Escherichia coli HB101 strain. The recombinant cells were immobilized in alginate beads and showed, similarly to the free cells, optimal temperatures for d-galactose and d-glucose isomerisation of 80 and 85 °C, respectively. The two isomerases were optimally active at pH 7.5. Cell entrapment significantly enhanced the acidotolerance of the two isomerases, as well as their stability at high temperatures. To perform simultaneous isomerisation of d-galactose and d-glucose at 65 °C and pH 7.5 in packed-bed bioreactor, cells concentration, dilution rate, productivity and bioconversion rate were optimized to be 32 g/l, 2.6 h−1, 3 g/l h and 30%, respectively.

Keywords
B, Bacillus; l-AI, l-arabinose isomerase; GI, glucose isomerase; HPLC, high performance liquid chromatography; HFCS, high fructose corn syrupl-Arabinose isomerase; Glucose isomerase; Co-expression; Immobilization; d-Fructose; d-Tagatose
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Co-expression of l-arabinose isomerase and d-glucose isomerase in E. coli and development of an efficient process producing simultaneously d-tagatose and d-fructose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 6, 2 May 2007, Pages 1531–1537
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us