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A composite enzymatic membrane for the specific separation and concentration of l glycerophosphate from a racemic d/l glycerophosphate mixture

Paper ID Volume ID Publish Year Pages File Format Full-Text
18254 42715 2007 4 PDF Available
Title
A composite enzymatic membrane for the specific separation and concentration of l glycerophosphate from a racemic d/l glycerophosphate mixture
Abstract

A composite enzymatic membrane (CEM), made up of two enzymatic membranes carrying two enzymes catalyzing two opposite reactions on either side of a permeable negatively charged membrane was used in this study. The phosphatase/kinase enzyme couple was chosen to make CEM permitting the active and selective transport of small-phosphorylated molecules. Here, we show that a CEM involving the alkaline phosphatase/glycerokinase enzyme couple permits, in a compartmentalized system, not only the separation of the d and l forms of a racemic mixture of (d/l) glycerophosphate, but also the simultaneous concentration of the l form alone.

Keywords
Enzymatic membranes; Active transports; Immobilized enzymes; Racemic separation
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A composite enzymatic membrane for the specific separation and concentration of l glycerophosphate from a racemic d/l glycerophosphate mixture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 6, 2 May 2007, Pages 1604–1607
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us