fulltext.study @t Gmail

Effects of dissolved carbon dioxide on growth, nutrient consumption, cephalosporin C synthesis and morphology of Acremonium chrysogenum in batch cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
18266 42716 2008 10 PDF Available
Title
Effects of dissolved carbon dioxide on growth, nutrient consumption, cephalosporin C synthesis and morphology of Acremonium chrysogenum in batch cultures
Abstract

Filamentous fungi are industrially important organisms because of their ability to produce secondary metabolites, and as expression systems for heterologous proteins. Since secretion of secondary metabolites in filamentous fungi is associated with the older, more vacuolated parts of the hyphae culture morphology is a crucial reflection of their physiology in submerged cultures. Therefore, any factor affecting fungal morphology will in turn affect secondary metabolite production. In this study we examine and report the effects of elevated levels of the potent morphogen, carbon dioxide upon morphology and antibiotic production in an industrially important fungal system. The effects of elevated CO2 levels on cephalosporin C (CPC) production, metabolic performance and morphological changes in batch cultures of Acremonium chrysogenum (ATCC 48272) were investigated. High levels of dCO2 led to reduction of CPC production by up to 36%. Both metabolic activity and substrate uptake were severely inhibited by elevated CO2, especially at 15 and 20% CO2. Both micro and macromorphology of A. chrysogenum were profoundly changed in bioprocesses sparged with CO2 especially at high levels, and these changes were a contributory cause of the reduction in CPC production.

Keywords
Filamentous fungi; Acremonium chrysogenum; Batch culture; dCO2; CPC; Morphology
First Page Preview
Effects of dissolved carbon dioxide on growth, nutrient consumption, cephalosporin C synthesis and morphology of Acremonium chrysogenum in batch cultures
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 42, Issue 4, 4 March 2008, Pages 315–324
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us