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Effect of macro/micro nutrients and carbon source over the denitrification rate of Haloferax denitrificans archaeon

Paper ID Volume ID Publish Year Pages File Format Full-Text
18280 42717 2007 9 PDF Available
Title
Effect of macro/micro nutrients and carbon source over the denitrification rate of Haloferax denitrificans archaeon
Abstract

The objective of the research project was to assess the impact of culturing parameters and medium composition on the capability of halophilic microorganisms to denitrify brines. Haloferax denitrificans archaeon were used for the denitrification process. Optimal temperature and pH values as well as NaCl concentrations were determined for the examined bacteria. Experiments were carried out according to the Latin square design. The performed investigations consisted in the selection of the medium which was to guarantee good conditions for the growth and denitrification for the examined bacteria. This medium differed from those employed for the non-halophilic denitrification bacteria and that is why it was necessary to supply the media with macroelements in the form of potassium (I), magnesium (II), and calcium (II) ions as well as such microelements as molybdenum (VI), iron (II), and copper (II). The above-mentioned components helped halophilic microorgansms survive in unfavourable environmental conditions characterised by a high osmotic potential.Methanol was selected as the carbon source for the denitrification and, in addition, the medium was supplemented with 250 mg dm−3 yeast extract which provided the experimental halophilic archaeon with a rich source of amino acids and active substances essential for their growth and development.

Keywords
Denitrification; Brine; Halophilic archaeon
First Page Preview
Effect of macro/micro nutrients and carbon source over the denitrification rate of Haloferax denitrificans archaeon
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 212–220
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering