fulltext.study @t Gmail

Optimisation of the biocatalytic resolution of styrene oxide by whole cells of Rhodotorula glutinis

Paper ID Volume ID Publish Year Pages File Format Full-Text
18281 42717 2007 7 PDF Available
Title
Optimisation of the biocatalytic resolution of styrene oxide by whole cells of Rhodotorula glutinis
Abstract

Various studies have described the optimisation of the hydrolysis of epoxides through epoxide hydrolase, however, far fewer have investigated the specific application of whole cells containing the enzyme. For this reason the enantioselective biocatalytic hydrolysis of styrene oxide by Rhodotorula glutinis UOFS Y-0653 through the use of whole cells was explored. It was found that a pH of 7.2, temperature of 45 °C and an initial substrate concentration of 50 mM led to maximum enzymatic activity. The whole cells were resistant to a changing environment. High temperatures were found to increase enzymatic activity but decrease enantioselectivity. At low temperatures (15 °C) enantioselectivity was significantly increased leading to an increase in both enantiopure substrate yield and the enantiomeric excess of both the substrate and product. No substrate inhibition was observed at initial substrate concentrations as high as 100 mM. The low deactivation energy (85.2 kJ/mol) obtained for this hydrolysis reaction suggests thermal instability of the enzyme. No significant effect on the reaction was observed when using unbuffered water instead of phosphate buffer as reaction medium.

Keywords
Yeast epoxide hydrolase; Enantioselectivity; Styrene oxide; Optimisation; Whole cells
First Page Preview
Optimisation of the biocatalytic resolution of styrene oxide by whole cells of Rhodotorula glutinis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 221–227
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us