fulltext.study @t Gmail

Hydroxypropyl-β-cyclodextrin induced complexation for the biocatalytic resolution of a poorly soluble epoxide

Paper ID Volume ID Publish Year Pages File Format Full-Text
18282 42717 2007 8 PDF Available
Title
Hydroxypropyl-β-cyclodextrin induced complexation for the biocatalytic resolution of a poorly soluble epoxide
Abstract

The use of a cyclodextrin induced inclusion complex can be very useful during the biohydrolysis of poorly soluble substrates. By using hydroxypropyl-β-cyclodextrin (HPB), the water solubility of styrene oxide (SO) was increased seven-fold (from 2.12 to 15.11 g/l at 15 °C) (20%, w/v) and at 10% (w/v) HPB SO was almost three times (9.71 g/l at 15 °C) more soluble than in 10% dimethylsulfoxide (DMSO) (w/v) (3.80 g/l at 15 °C). Increasing concentrations of HPB did not influence the enzymatic stability of epoxide hydrolase (EH) from Rhodotorula glutinis contrary to the organic co-solvents which negatively influenced it in the order DMSO > dimethylformamide (DMF). HPB does, however, negatively influence the EH activity of the biocatalyst R. glutinis UOFS Y-0653. When compared to the inhibition resulting from the addition of organic co-solvents, the inhibition was in the order DMF > HPB > DMSO. Inactivation constants of, 4.54 ± 0.32, 3.29 ± 0.28 and 1.81 ± 0.13 (weight ratio−1 w/v) were determined for DMF, HPB and DMSO, respectively. Neither DMSO nor DMF influenced the selectivity of the reaction while HPB decreased it slightly. Thermal studies yielded inactivation energy (Ea) values of 84.0, 82.3 and 86.0 kJ/mol for DMSO, DMF and HPB, respectively.

Keywords
Biocatalytic resolution; Cyclodextrin; Co-solvents; DMF; DMSO; Solubility; Styrene oxide
First Page Preview
Hydroxypropyl-β-cyclodextrin induced complexation for the biocatalytic resolution of a poorly soluble epoxide
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 228–235
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us