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Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film

Paper ID Volume ID Publish Year Pages File Format Full-Text
18287 42717 2007 4 PDF Available
Title
Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film
Abstract

The steady state amperometric measurements of free cholesterol were performed by an enzyme electrode which was developed through electrostatic immobilization of the cholesterol oxidase in poly(vinylferrocenium) perchlorate, PVF+ClO4−, film that was coated on a Pt electrode. Amperometric measurements were performed at an applied potential of 0.7 V versus SCE. The effects of enzyme concentration, polymer film thickness, working temperature, dissolved oxygen gas and pH of solution on the response electrode were elucidated. The activation energy and the apparent Michaelis–Menten constant were calculated for enzyme electrode system. The linear working range, the sensitivity and the stability of the biosensor were also determined. The mechanism for the operation of the enzyme electrode was proposed.

Keywords
Amperometric enzyme electrode; Cholesterol oxidase; Free cholesterol biosensor; Poly(vinylferrocenium)
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Amperometric enzyme electrode for free cholesterol determination prepared with cholesterol oxidase immobilized in poly(vinylferrocenium) film
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 262–265
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us