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Regioselective formation of kojic acid-7-o-alpha-d-glucopyranoside by whole cells of mutated Xanthomonas campestris

Paper ID Volume ID Publish Year Pages File Format Full-Text
18296 42717 2007 5 PDF Available
Title
Regioselective formation of kojic acid-7-o-alpha-d-glucopyranoside by whole cells of mutated Xanthomonas campestris
Abstract

Whole cells of mutated Xanthomonas campestris were successfully used as a biocatalyst for the glucosylation of kojic acid. Suitable carbon and nitrogen sources and the pH for the cultivation of the mutated cells at 30 °C were identified for maximum conversion. Using 0.1 g of the cells, 80% conversion was obtained after 28 h at 30 °C and by using maltose as the acyl donor at a molar ratio of 1:8. Under the same conditions, the use of wild type cells resulted in 13% conversion only. 1D NMR and 2D shift-correlated NMR (1H × 1H-COSY, 1H × 13C-COSY) analysis showed that the product was kojic acid 7-o-alpha-d-glucopyranoside.

Keywords
Whole cells; Xanthomonas campestris; Kojic acid glucoside; Glucosylation
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Regioselective formation of kojic acid-7-o-alpha-d-glucopyranoside by whole cells of mutated Xanthomonas campestris
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 324–328
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us