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Analysis of novel hemoglobins during microaerobic growth of HMP-negative Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
18297 42717 2007 8 PDF Available
Title
Analysis of novel hemoglobins during microaerobic growth of HMP-negative Escherichia coli
Abstract

Limited oxygen availability is a prevalent problem in microbial biotechnology leading to reduced growth and productivity. Vitreoscilla hemoglobin (VHb) has been extensively studied showing positive and energetically favorable characteristics. Species-specific differences also suggest that VHb is not optimal for all applications. Therefore, mutant and fusion proteins were generated with improved characteristics. Hemoglobins of various microorganisms also provide phenotypes eliciting positive properties. Therefore, two new globin genes, encoding Novosphingobium aromaticivorans hemoglobin (NHb) and Bacillus halodurans flavohemoglobins (HmpBh), were isolated. Genes encoding two mutant flavohemoglobins of Pseudomonas aeruginosa PAO1 and a clinical isolate were also analyzed. The proteins were expressed to study the physiological effects of globin expression relative to controls, carrying either the parental plasmid or expressing VHb, during oxygen limited fed-batch cultivations of Escherichia coli BO6, lacking the endogenous flavohemoglobin. HmpBh-expressing cells reached 25 and 10% higher final optical densities relative to the plasmid carrying or VHb-expressing cells, respectively. HmpBh-expressing cells also showed 34% higher yield of biomass on glucose relative to control. Sequence comparison indicates that hemoglobin domains of Bacillus species are closely related to VHb sequence. Thus, suggesting that proteins having a globin domain similar to VHb could be important in biotechnology.

Keywords
FHP, FlavoHb of Ralstonia eutropha; FlavoHb(s), flavohemoglobin(s); Hb(s), hemoglobin(s); HmpBh, Bacillus halodurans; FlavoHb, hmpBh flavohb gene of Bacillus halodurans; HmpCPA10, FlavoHb of Pseudomonas aeruginosa CPA10, a clinical isolate from urine; hmp
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Analysis of novel hemoglobins during microaerobic growth of HMP-negative Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 329–336
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us