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Improved stabilization of microencapsulated Cathepsin B in harsh conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
18298 42717 2007 6 PDF Available
Title
Improved stabilization of microencapsulated Cathepsin B in harsh conditions
Abstract

A cysteine protease Cathepsin B purified from goat brain was microencapsulated within the CM-cellulose-Ca-alginate membrane having a liquid core in it. The activity yield was maximum under the optimum conditions of immobilization mainly at 4% CM-cellulose, 1% CaCl2 and 3% alginate concentrations. The encapsulated form of enzyme could retain ∼60% of its original activity during its reusability for more than five successive batch reactions. The physico-chemical characteristics for free and immobilized Cathepsin B were also compared. The optimum pH and temperature of free and immobilized enzyme were 6.0 and 50 °C, and 6.0–6.5 and 55 °C, respectively. The K′mK′m value (1.52 mM) for the immobilized enzyme was one and half-fold higher than for the soluble enzyme. The pH and thermal stability as well as organic solvent stability were improved significantly after microencapsulation may make it useful for catalyzing some organic reactions like trans-esterification and trans-amidation.

Keywords
Microencapsulation; Cysteine protease; Cathepsin B; Ethanol and activity yield
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 337–342
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us