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Disk-shaped immobilized multicomponent reagent for bioluminescent analyses: Correlation between activity and composition

Paper ID Volume ID Publish Year Pages File Format Full-Text
18299 42717 2007 4 PDF Available
Title
Disk-shaped immobilized multicomponent reagent for bioluminescent analyses: Correlation between activity and composition
Abstract

A disk-shaped reagent based on bacterial NADH:FMN-oxidoreductase and luciferase immobilized into a starch gel is developed. The reagent characteristics, such as activity and stability depend on the time of drying, the initial luciferase preparation content, the source of the starch and its gel concentration. The experimental error of bioluminescence determination does not exceed 10–15%. The method of immobilization into gel provides the coimmobilization luciferase and NADH:FMN-oxidoreductase and their substrates. On the basis of these reagents, the multifunctional immobilized biosensor for bioluminescent assay in medicine, ecology and other fields may be developed.

Keywords
L, bacterial luciferase; R, NADH:FMN-oxidoreductase; FMN, oxidized form of flavin mononucleotide; NADH, reduced nicotineamine adeninedinucleotide; C14, myristic aldehydes, tetradecanal; DTT, dithiothreitolBioluminescence; Bacterial luciferase; NADH:FMN-ox
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Disk-shaped immobilized multicomponent reagent for bioluminescent analyses: Correlation between activity and composition
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 343–346
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us