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Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides

Paper ID Volume ID Publish Year Pages File Format Full-Text
18301 42717 2007 8 PDF Available
Title
Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides
Abstract

Among several fruit seeds, apple seed was identified as a new promising β-glucosidase source for alkyl O-glucoside synthesis by reverse hydrolysis, since it showed high hydrolytic activities on a broad spectrum of β-glucosides. From the crude extract of apple seed meal, a major glucosidase isoenzyme was purified to homogeneity, with a purification factor of 47-fold and an overall yield of 12.8%, by ammonium sulfate fractionation and chromatographic separation through DEAE-Cellulose, Butyl-Toyopearl and Sephadex G150 columns. The purified enzyme is a homodimer; each subunit has a molecular mass of about 60 kDa as determined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the pI of this β-glucosidase is about 5.7. These properties indicate that the apple seed β-glucosidase is totally different from any of the almond β-glucosidase isozymes reported so far. Furthermore, the purified enzyme of apple seed displays higher thermal stability than the commercially supplied β-glucosidase from almond, with half-lives of 42.9 h and 14.2 h, respectively, as preserved at 50 °C in an aqueous environment. The optimum pH of the apple seed β-glucosidase was 6.0 and the stable pH range was 5.0–9.0, both similar to those of the commercial almond β-glucosidase.

Keywords
Apple seed; Characterization; β-Glucosidase; Purification; Thermal stability
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Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 40, Issue 2, 4 January 2007, Pages 354–361
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us