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Esculin gel diffusion assay (EGDA): A simple and sensitive method for screening β-glucosidases

Paper ID Volume ID Publish Year Pages File Format Full-Text
18309 42718 2006 3 PDF Available
Title
Esculin gel diffusion assay (EGDA): A simple and sensitive method for screening β-glucosidases
Abstract

β-Glucosidase is an important component of the cellulose degrading enzyme system. The enzyme has other biological applications as well. Often a large number of samples have to be screened for β-glucosidase activity, such as fractions obtained from column chromatography or environmental samples. A gel diffusion technique is presented for screening any number of samples with less labour and cost as compared to other screening methods used. The technique is based on the well-established reaction of β-glucosidase with esculin. Chromatographic fractions were successfully screened to the same degree of accuracy as one of the most commonly used p-nitrophenyl-β-d-glucopyranoside assay.

Keywords
β-Glucosidase; Screening; Gel diffusion assay; Esculin
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Esculin gel diffusion assay (EGDA): A simple and sensitive method for screening β-glucosidases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 2, 26 June 2006, Pages 182–184
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us