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Dissolved carbon dioxide effects on growth, nutrient consumption, penicillin synthesis and morphology in batch cultures of Penicillium chrysogenum

Paper ID Volume ID Publish Year Pages File Format Full-Text
18310 42718 2006 6 PDF Available
Title
Dissolved carbon dioxide effects on growth, nutrient consumption, penicillin synthesis and morphology in batch cultures of Penicillium chrysogenum
Abstract

Dissolved carbon dioxide is one variable that changes between scales of bioreactor and may thus have a potential influence on the process as scale alters, and especially so in filamentous fungal cultures. The effects of dCO2 on growth and penicillin production by an industrial strain of Penicillium chrysogenum were examined systematically using a steam sterilisable dCO2 probe and a computerized image analysis system. Although low levels of CO2 stimulated growth (increased biomass, lower mean hyphal growth unit (M.H.G.U.) in lag phase) at the expense of antibiotic synthesis, higher levels led to elevated M.H.G.U.'s in the lag phase and were associated with increased clumping and severely reduced penicillin titre. The adverse effects of dCO2 in these cultures were largely mediated by inhibition of growth and substrate uptake in the lag phase, causing morphological changes which might also contribute to reduced penicillin levels.

Keywords
Filamentous fungi; Morphology; Product formation; Carbon dioxide; Image analysis
First Page Preview
Dissolved carbon dioxide effects on growth, nutrient consumption, penicillin synthesis and morphology in batch cultures of Penicillium chrysogenum
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 2, 26 June 2006, Pages 185–190
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering