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Tyrosol to hydroxytyrosol biotransformation by immobilised cell extracts of Pseudomonas putida F6

Paper ID Volume ID Publish Year Pages File Format Full-Text
18311 42718 2006 6 PDF Available
Title
Tyrosol to hydroxytyrosol biotransformation by immobilised cell extracts of Pseudomonas putida F6
Abstract

Cell extracts of Pseudomonas putida F6 expressing tyrosinase activity were immobilised in a calcium alginate matrix. Immobilised cell extracts consumed 21% more tyrosol substrate (2.5 mM) than non-immobilised cell extracts of P. putida F6. However, approximately the same level of hydroxytyrosol accumulation was observed. A number of approaches were attempted for complete removal of tyrosol substrate and maximum hydroxytyrosol accumulation. These approaches included addition of ascorbic acid, washing and re-use of immobilised cell extracts, sequential addition of fresh immobilised cell extracts to the biotransformation, and alteration of the starting concentration of tyrosol. Complete substrate removal was observed in the presence of both 1.0 and 2.5 mM tyrosol with equimolar concentrations of ascorbic acid. The sequential addition of immobilised cell extracts was required to ensure complete removal of 2.5 mM substrate. However, only 6% of the 2.5 mM substrate accumulated as product (hydroxytyrosol). The biotransformation of 1 mM tyrosol resulted in the accumulation of 0.8 mM hydroxytyrosol in a single biotransformation with immobilised cell extracts. Ninety-six percent of the hydroxytyrosol was subsequently recovered from the biotransformation reaction using an Amberlite XAD-4® resin. Immobilised cell extracts retained 58% of their activity after 9 days of storage on filter paper at 4 °C while cell free extracts retained only 22% of their activity under the same storage conditions.

Keywords
Pseudomonas putida F6; Tyrosol; Hydroxytyrosol
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Tyrosol to hydroxytyrosol biotransformation by immobilised cell extracts of Pseudomonas putida F6
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 2, 26 June 2006, Pages 191–196
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us