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Observation and modeling of induction effect on human transferrin production from stably transfected Drosophila S2 cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
18313 42718 2006 7 PDF Available
Title
Observation and modeling of induction effect on human transferrin production from stably transfected Drosophila S2 cell culture
Abstract

Human transferrin (hTf) is a serum glycoprotein involved in iron transport. We performed, for the first time, mathematical modeling of stably transfected insect Drosophila melanogaster S2 cell culture, a nonlytic plasmid-based system that secretes recombinant hTf under control of the copper sulfate-inducible Drosophila metallothionein promoter. Cell growth patterns at various inducer concentrations revealed that the specific growth rate of S2 cells was substantially reduced as the specific rate of recombinant hTf production increased, and recovered to some extent when recombinant hTf production was nearly stopped. Additionally, the time profiles of specific production rates exhibited a maximum in the early culture period. Longer times and lower values of the maximum specific production rate were observed at lower inducer concentrations, and shorter times and higher values at higher inducer levels. Although the proposed S2 cell culture model was slightly limited with regard to prediction of cell growth profile at the late stage, we ensured that it gave reasonable predictions of the dynamics of glucose consumption and recombinant hTf production and confirmed its validity through simulating other culture experiments under different conditions. The S2 model proposed in this study can contribute to the elucidation of cell culture dynamics, and optimization of each culture variable to enhance heterologous protein production.

Keywords
Insect cell culture; Drosophila S2 cells; Modeling; Simulation; Optimization; Human transferrin
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Observation and modeling of induction effect on human transferrin production from stably transfected Drosophila S2 cell culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 2, 26 June 2006, Pages 208–214
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us