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Synthesis of p-hydroxyphenylglicine by cell extract from Agrobaterium radiobacter encapsulated in alginate capsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
18314 42718 2006 7 PDF Available
Title
Synthesis of p-hydroxyphenylglicine by cell extract from Agrobaterium radiobacter encapsulated in alginate capsules
Abstract

A crude cell extract from Agrobacterium radiobacter was encapsulated in calcium-alginate capsules for the synthesis of p-hydroxyphenylglicine (p-HPG). The effect of the encapsulation process on the two enzymatic activities involved on the p-HPG synthesis was studied. The encapsulation process was optimised with regard to alginate concentration and ratio extract:alginate. The encapsulation efficiency was around a 60% and the derivatives showed a p-HPG synthesis yield of around a 50%. The optimum temperature was 55 °C and the activation energy (Ea) of the whole process was 4.4 kcal/mol. The biocatalyst could be used six times without any activity loss.

Keywords
p-Hydroxyphenylglicine; Encapsulation; d-Hydantoinase; N-carbamyl-d-amino acid amydohydrolase; Alginate
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Synthesis of p-hydroxyphenylglicine by cell extract from Agrobaterium radiobacter encapsulated in alginate capsules
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 2, 26 June 2006, Pages 215–221
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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