fulltext.study @t Gmail

Production of androstenones from phytosterol by mutants of Mycobacterium sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
18324 42718 2006 5 PDF Available
Title
Production of androstenones from phytosterol by mutants of Mycobacterium sp.
Abstract

An androst-4-ene-3,17-dione (AD)-producing mutant, AD1 and an androsta-1,4-diene-3,17-dione (ADD)-producing mutant, PD3 were derived from a cholesterol-, phytosterol-assimilating bacterium Mycobacterium sp. B-3805S and a cholesterol-assimilating bacterium Mycobacterium sp. NRRL B-3683, respectively, using nitrosoguanidine (NTG) mutagenesis. Production of AD or ADD from phytosterol by the two mutants was investigated in a 5-L surface-aeration microprocessor-controlled fermentor at 30 °C. It was found that the maximum conversion of phytosterol to AD or ADD was 67.9 or 70.6%, respectively. Control of glucose concentration in the fermentation culture is an important parameter that affects production of AD or ADD as a sole product from phytosterol. The major components of phytosterol such as campesterol, stigmasterol and β-sitosterol were proportionally consumed by the two mutants without nutrient preference. AD or ADD were isolated from the fermentation broth by the addition of Amberlite XAD-7 resin and were further purified by silica gel column chromatography. After crystallization in methanol, the purified AD or ADD was obtained as needle crystals, which were identical to authentic AD or ADD by physical and instrumental analyses.

Keywords
Androstenone; Androst-4-ene-3,17-dione; Androsta-1,4-diene-3,17-dione; Phytosterol; Mycobacterium
First Page Preview
Production of androstenones from phytosterol by mutants of Mycobacterium sp.
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 2, 26 June 2006, Pages 296–300
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us