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Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production

Paper ID Volume ID Publish Year Pages File Format Full-Text
18336 42719 2006 7 PDF Available
Title
Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production
Abstract

The hyoscyamine 6β-hydroxylase (H6H) gene of plant origin and 4-hydroxycinnamoyl-CoA hydratase/lyase (HCHL) gene of bacterial origin were independently introduced into the tree plant Duboisia leichhardtii using Agrobacterium rhizogenes as a vector to enhance scopolamine and reduce lignin production, respectively. H6H and HCHL gene positive as well as negative hairy root clones were analyzed for their expression by RT-PCR. No expression of HCHL was detected in any clone, whereas expression of the exogenous H6H gene was distinguished from the endogenous gene by detection of an amplified untranslated region of the promoter. No difference in root morphology between H6H and HCHL positive clones was observed, but the alkaloid profiles were different between both clones. Hyoscyamine was more potently produced than scopolamine in HCHL gene positive clones and reflecting the absence of gene expression, there was not much variation among the clones. On the other hand, H6H positive clones displayed highly variable alkaloid spectra, depending on exogenous H6H gene expression. The best clone yielded a greater than 95% conversion rate from hyoscyamine to scopolamine.

Keywords
Duboisia leichhardtii; Hairy root; Hyoscyamine 6β-hydroxylase (H6H); 4-hydroxycinnamoyl-CoA hydratase/lyase (HCHL); Scopolamine; Hyoscyamine
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Exogenous plant H6H but not bacterial HCHL gene is expressed in Duboisia leichhardtii hairy roots and affects tropane alkaloid production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1183–1189
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us