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Enzymatic immobilization of 2,4,6-trinitrotoluene (TNT) biodegradation products onto model humic substances

Paper ID Volume ID Publish Year Pages File Format Full-Text
18338 42719 2006 8 PDF Available
Title
Enzymatic immobilization of 2,4,6-trinitrotoluene (TNT) biodegradation products onto model humic substances
Abstract

The ability to enzymatically immobilize TNT biotransformation metabolites onto humic monomers was investigated using new laccase producing fungal isolates, namely: Trametes modesta BT2368, Trametes versicolor BT2570, Trametes hirsuta BT2566, T. hirsuta BT2133 and Sclerotium rolfsii CBS 350.80. Degradation of TNT by these organisms was confirmed using HPLC and it was shown to lead predominantly to biotransformation. Aminodinitrotoluenes (4-aminodinitrotoluene (4-AMDNT) and 2-aminodinitrotoluene (2-AMDNT)) were identified as the major biotransformation products. T. hirsuta BT2566 cultures supplemented with 220 μM TNT produced the highest amounts of 4-AMDNT and 2-AMDNT up to a concentration of 104 and 52 μM after 96 h of incubation, respectively. Incorporation of 200 mM of humic monomers especially ferulic acid, guaiacol and catechol during the biotransformation of TNT drastically reduced the accumulation of aminodinitrotoluenes to below 30%. In the presence of a purified laccase from T. hirsuta complete immobilization of 2-hydroxylaminodinitrotoluenes (2-HADNT) and 4-hydroxylaminodintrotoluenes (4-HADNT) by the different humic monomers was achieved. Ferulic acid was the most effective humic monomer in immobilizing TNT metabolites both in in vitro and culture experiments.

Keywords
2,4,6-Trinitrotoluene; Biodegradation; Biotransformation; Fungi; Immobilization; Humic monomers
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Enzymatic immobilization of 2,4,6-trinitrotoluene (TNT) biodegradation products onto model humic substances
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1197–1204
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us