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Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
18341 42719 2006 7 PDF Available
Title
Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate
Abstract

Production of protease by a local wild-type strain, Aspergillus terreus IMI 282743 using pre-filtered palm oil mill effluent (POME) and its derivatives was investigated. POME was first subjected to depth and surface filtration to remove the coarse solids found in the wastewater. Then, the pre-filtered POME was concentrated twofold to form retentate by means of ultrafiltration process. Maximum protease activity of 129 U/ml was achieved on the fourth day of fermentation in the medium containing 75% (v/v) retentate of pre-filtered POME as the sole carbon and nitrogen sources, operated at 37 °C, 250 rpm and 5% (v/v) of inoculum concentration. The results indicated that addition of nutrients and adjustment of initial pH were dispensable in this particular study to obtain better yield of protease. On the other hand, a decrease of about 59% and 19% of maximum protease activity found in the medium of pure retentate (100%, v/v) compared to the medium of 75% (v/v) and 50% (v/v) retentate, respectively, suggesting that retentate should be diluted prior to the fermentation process in order to gain better yield of protease.

Keywords
Ultrafiltration; Retentate; Aspergillus terreus; Protease; Palm oil mill effluent (POME)
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Investigations on protease production by a wild-type Aspergillus terreus strain using diluted retentate of pre-filtered palm oil mill effluent (POME) as substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1223–1229
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us