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Direct acylation of flavonoid glycosides with phenolic acids catalysed by Candida antarctica lipase B (Novozym 435®)

Paper ID Volume ID Publish Year Pages File Format Full-Text
18343 42719 2006 6 PDF Available
Title
Direct acylation of flavonoid glycosides with phenolic acids catalysed by Candida antarctica lipase B (Novozym 435®)
Abstract

Immobilised Candida antarctica lipase B (Novozym 435®) was used to acylate flavonoid glycosides, both purified and in fruit extracts, using carboxylic acids as acyl donors, in the presence of 4 Å molecular sieves, using 2-methyl-2-propanol as solvent. Conversions ranged from ∼25% to ∼95% using palmitic, cinnamic and phenylpropionic (PPA) acids and hydroxylated derivatives of PPA as acyl donors. Both naringin (naringenin-7-rhamnoglucoside) and isoquercetin (quercetin-3-O-glucopyranoside) were readily acylated. Both compounds gave a major mono-acylated product, along with minor mono-acylated products, which were presumably positional isomers and also di-acylated products. When apple or blueberry extracts were the source of flavonoids, the enzyme showed strong selectivity for acylation of glycosides with a primary aliphatic hydroxyl group on the sugar moiety, such as phloridzin (phloretin-2-glucoside) and anthocyanidin glucosides and galactosides. No acylation of compounds lacking a primary aliphatic hydroxyl group, such as flavonoid aglycones, chlorogenic acid (caffeoyl quinate) or anthocyanidin arabinosides was observed. Acylation, particularly with palmitic acid, greatly increased lipophilicity and solubility in the reaction solvent.

Keywords
Lipase; Enzymic acylation; Flavonoid; Polyphenolic; Phenolic acid
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Direct acylation of flavonoid glycosides with phenolic acids catalysed by Candida antarctica lipase B (Novozym 435®)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1236–1241
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us