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Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators

Paper ID Volume ID Publish Year Pages File Format Full-Text
18344 42719 2006 8 PDF Available
Title
Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators
Abstract

The laccase produced by the white-rot fungus Pleurotus ostreatus D1 (BLPO) under submerged cultivation was purified to homogeneity. Its catalytic activity towards several phenolic compounds, including ABTS (2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt), syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehyde azine), 2,6-dimethoxyphenol and catechol was investigated. pH-optima and kinetic constants (Km and Vmax) of these reactions were determined. The enzyme was able to degrade the following polyaromatic hydrocarbons (PAHs): anthracene (91%), phenanthrene (72%), fluorene (53.5%), pyrene (65.5%), fluoranthene (69.7%), and perylene (73%) only in the presence of a synthetic mediator. The degradation of phenanthrene in the presence of detergents varied from 49% to 72%, whereas in the absence of any detergent it reached 10%. Investigating the effect of various mediators on PAHs degradation by the BLPO showed that ABTS was a better mediator of anthracene oxidation and HBT (1-hydroxybenzotriazole) was a better mediator of fluorene oxidation. Pyrene and anthracene were degraded more rapidly in a mixture than separately. The effect of different solvents on the activity of the BLPO towards anthracene and fluorene was investigated also. Finally, it was found that in a solution of 30% (v/v) acetonitrile, BLPO could degrade about 56% of the 600 μM initially added anthracene with the formation of anthraquinone.

Keywords
BLPO, blue laccase from Pleurotus ostreatus D1; PAHs, polycyclic aromatic hydrocarbons; ABTS, 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt; syringaldazine, 4-hydroxy-3,5-dimethoxybenzaldehyde azine; DMOP, 2,6-dimethoxyphenol; A
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Oxidative degradation of polyaromatic hydrocarbons catalyzed by blue laccase from Pleurotus ostreatus D1 in the presence of synthetic mediators
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Publisher
Database: Elsevier - ScienceDirect
Journal: Enzyme and Microbial Technology - Volume 39, Issue 6, 3 October 2006, Pages 1242–1249
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us